| Abstrakt: |
[Objectives] This study was conducted to observe the mechanism of Dendrobium officinale Kimura et Migo on gastrocnemius muscle in rats with exercise-induced muscle damage (EIMD). [Methods] The micro-injury model of skeletal muscle was established by treadmill training. Forty two SD rats were randomly divided into a control group, 1, 12 and 24 h exercise groups, D. officinale 2 ml 4-1 h exercise group, D. officinale 2 ml 4-12 h exercise group, and D. officinale 2 ml 4-24 h exercise group, with 6 rats in each group. Various D. officinale groups were given the drug once in the morning and once in the evening at a dose of 2 ml/time, a week in advance. Except for the quiet group, the samples were collected from the 1, 12 and 24 h exercise groups after anesthesia following 1, 12 and 24 h of exercise for the last time, respectively, and the D. officinale 2 ml 4-1 h exercise group, D. officinale 2 ml 4-12 h exercise group and D. officinale 1 ml 4-24 h exercise group were also sampled after anesthesia following 1, 12 and 24 h of exercise for the last time, respectively. The contents of ATP, CK-MM and CK in rat serum were determined by enzyme-linked immunosorbent assay (ELISA). The histopathological changes of gastrocnemius muscle were observed by HE staining. PCR and Westem-blot detection were carried out to analyze the effects of D. officinale on IGF-1 mRNA and protein expression in gastrocnemius muscle. [Results] Compared with the quiet group, the ATP contents in the serum of rats in the 1, 12 and 24 h exercise groups significantly decreased (P<0.01), while the CK and CK-MM contents significantly increased (P<0. 01). The expression of IGF-1 mRNA and protein in the gastrocnemius muscle tissue significantly increased (P<0. 01). Compared with the 1 h exercise group, the ATP content and IGF-1 protein expression in the gastrocnemius muscle tissue of the D. officinale liquid 4-1 h exercise group significantly increased (P <0.05), while the CK and CK-MM contents significantly decreased (P <0. 01). Compared with the 12 h exercise group, the D. officinale liquid 4-12 h exercise group showed a significant increase in ATP content (P <0. 01), significant increases in IGF-1 mRNA and protein expression in the gastrocnemius muscle tissue (P <0. 01), and significant decreases in CK and CK-MM contents (P <0. 01). Compared with the 24 h exercise group, the ATP content and IGF-1 mRNA and protein expression in the gastrocnemius muscle tissue of the D. officinale liquid 4-24 h exercise group significantly increased (PcO.Ol), while the CK and CK-MM contents significantly decreased (P <0. 01). From the pathological tissue morphology of the gastrocnemius muscle in rats with EIMD treated with D. officinale, it could be concluded that the gastrocnemius muscle of each exercise group was significantly damaged, and the damage was significantly alleviated after administration of D. officinale liquid. [Conclusions] The effects and mechanism of D. officinale on prevention and treatment of EIMD in rats might be related to the promotion of IGF-1 mRNA and protein expression in injured tissues by reducing ATP energy consumption, CK-MM and CK activity. [ABSTRACT FROM AUTHOR] |
