| Abstrakt: |
Tamarix chinensis Lour. is native to China and has characteristics such as heat resistance and salt alkali resistance. It is mostly distributed in coastal or inland desert areas of China. In this article, 36 samples of Tamarix chinensis collected from the Bohai Rim region (11 samples from Shandong, 5 samples from Tianjin, 15 samples from Hebei, and 5 samples from Liaoning) were sequenced by specific locus amplified fragment sequencing (SLAF-seq). Based on the willow genome as a reference, the system design of the experimental scheme was carried out by bioinformatics analysis. The specific length of DNA fragments was screened, and the SLAF-seq library was constructed. A large number of sequences were obtained by high-throughput sequencing. Then, polymorphic SLAF tags were obtained by software analysis and comparison. Finally, a large number of specific single nucleotide polymorphisms (SNPs) were developed on the polymorphic SLAF tags. A total of 2,341,663 SLAF tags were obtained, including 51,985 polymorphic SLAF tags. All SNPs were filtered according to integrity > 0.5 and MAF > 0.05, and 157,821 highly consistent SNPs were obtained. The SNPs were analyzed by statistical methods, and 36 Tamarix samples from four regions were obtained. Phylogenetic tree, population structure, and principal component analyses were completed, which revealed the genetic differentiation between individuals at the genome level. The results showed that the Tamarix chinensis from the four regions all originated from the same ancestor, but due to the different growth environment and geographical location, genetic differentiation occurred in the process of growth and development. However, the genetic differences among the individuals were small. Through cluster analysis, clustering phenomenon was observed for some varieties. [ABSTRACT FROM AUTHOR] |
Full text from SpringerLink