Expanding the Anti-Phl p 7 Antibody Toolkit: An Anti-Idiotype Nanobody Inhibitor.

Autor:	Vester, Susan K., Davies, Anna M., Beavil, Rebecca L., Sandhar, Balraj S., Beavil, Andrew J., Gould, Hannah J., Sutton, Brian J., McDonnell, James M.
Předmět:	RECOMBINANT antibodies MONOCLONAL antibodies IMMUNOGLOBULINS CRYSTAL structure ANTIGENS
Zdroj:	Antibodies (2073-4468); Dec2023, Vol. 12 Issue 4, p75, 12p
Abstrakt:	We have previously produced a toolkit of antibodies, comprising recombinant human antibodies of all but one of the human isotypes, directed against the polcalcin family antigen Phl p 7. In this work, we complete the toolkit of human antibody isotypes with the IgD version of the anti-Phl p 7 monoclonal antibody. We also raised a set of nanobodies against the IgD anti-Phl p 7 antibody and identify and characterize one paratope-specific nanobody. This nanobody also binds to the IgE isotype of this antibody, which shares the same idiotype, and orthosterically inhibits the interaction with Phl p 7. The 2.1 Å resolution X-ray crystal structure of the nanobody in complex with the IgD Fab is described. [ABSTRACT FROM AUTHOR]
Databáze:	Complementary Index
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