| Abstrakt: |
Purpose: The present research aimed to describe a novel ciprofloxacin HCl-loaded bovine serum albumin nanoparticle formulation to improve the antibacterial potential of ciprofloxacin HCl for the treatment of recurrent urinary tract infections. Methods: Ciprofloxacin HCl-loaded bovine serum albumin nanoformulations were prepared using the desolvation method. The effects of formulation variables on particle size and distribution, zeta potential, and drug encapsulation were studied using 32-full factorial design. Results: The optimal formulation composed of a 2% (w/v) concentration of bovine serum albumin solution at pH 8.60 was successfully prepared. The predicted responses for the optimized ciprofloxacin HCl-loaded bovine serum albumin nanoparticles were found to be paralleled by the experimentally obtained 238.2 nm particle size, −27.6 mV zeta potential, 38.11% encapsulation efficiency, as well as a lower polydispersity index. The optimized formulation was also evaluated with respect to the in vitro drug release rates, morphological properties, and antibacterial activity. The antibacterial effect of the optimized formulation and its blank counterpart was assessed using conventional agar diffusion and broth microdilution method against Escherichia coli, Enterococcus faecalis, Staphylococcus aureus, and Pseudomonas aeruginosa. Conclusion: The results revealed that the optimized ciprofloxacin HCl-loaded albumin nanoparticle formulation exhibited improved antibacterial efficacy against microorganisms. [ABSTRACT FROM AUTHOR] |
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