Establishing morphological and molecular evidences to support the identification of "yerba buena" (Mentha × villosa Huds.) in the Philippines.

Autor: Quiñones, Kristine Joyce O., Gentallan Jr., Renerio P., Bartolome, Michael Cedric B., Madayag, Roselle E., Timog, Emmanuel Bonifacio S., Cruz, Juan Rodrigo A. Vera, Coronado, Nadine B., Endonela, Leah E., Borromeo, Teresita H., Altoveros, Nestor C., Alvaran, Bartimeus Buiene S., Cejalvo, Reneliza D.
Zdroj: Journal of Crop Science & Biotechnology; Dec2023, Vol. 26 Issue 5, p607-613, 7p
Abstrakt: Mentha × villosa is a perennial, aromatic herb that is commonly cultivated as a medicinal plant in the Philippines with verified medicinal properties. Using Illumina sequencing data, we assembled and annotated the complete chloroplast genome of an accession of M. × villosa (ICROPS 2020053) from the germplasm collection of the Institute of Crop Science, University of the Philippines Los Baños Laguna. Morphological characterization was done to ensure that the observed traits fall within the taxonomic description of the species and agree with the reference specimen conserved in the genebank and preserved in the herbarium. The complete cp genome is 152,048 bp long which exhibits a quadripartite circular structure and is characterized by a large single-copy of 83,179 bp, short single-copy of 17,653 bp, and a pair of inverted repeat regions of 25,068 bp each. The overall GC content of the chloroplast genome was 37.8%. The plastome is comprised of 130 genes coding for 37 tRNA, 8 rRNA and 84 mRNA. Phylogenetic analysis showed that M. × villosa is closely related to M. × piperita and M. spicata. This information further supports the conserved nature of chloroplast genomes despite historical natural interspecific hybridization. The assembled complete chloroplast genome of M. × villosa is valuable in elucidating phylogenetic relationships among Mentha species in the Philippines. These observed traits could be used as markers to identify M. × villosa, the medicinal species used by the Philippine herbal industry, which is often confused with other Mentha species. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index