Immulina as an Immunostimulatory Supplement: Formulation and Pharmacological Studies.

Autor: Li, Yusheng, Ji, Nan, Wang, Minia, Pugh, Nirmal D., Khan, Ikhlas A., Tan, Chalet
Předmět:
Zdroj: Planta Medica; Dec2023, Vol. 89 Issue 15, p1483-1492, 10p
Abstrakt: Immulina is a commercially available extract of Arthrospira platensis enriched with bacterial lipoproteins that acts as a potent Toll-like receptor 2 agonist. However, the immunostimulatory effect of Immulina is not well understood in vivo. Here, to devise an Immulina formulation suitable for in vivo oral gavage dosing, Immulina nanosuspension was prepared and freeze-dried to yield lyophilized nano-Immulina, which had an average particle size of around 300 nm and fully retained the bioactivity as a Toll-like receptor 2 agonist. Compared to the regular Immulina powder, lyophilized nano-Immulina notably accelerated the dissolution in aqueous media. Immulina nanosuspension was found to stimulate the production of proinflammatory cytokines in murine bone marrow-derived dendritic cells and macrophages. The immune response to Immulina was investigated in healthy mice by longitudinally monitoring the phagocytic activity of circulating neutrophils as a surrogate marker. Following daily oral ingestion of Immulina nanosuspension (10 mg/mouse/day), the phagocytic activity of circulating neutrophils was significantly elevated, suggesting an important mechanism for Immulina to enhance innate immunity. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index