| Abstrakt: |
Morinda reticulata Gamble possesses a substantial amount of polyphenolic antimicrobial compounds that can be used for controlling the growth of pathogenic microorganisms. The purpose of this study was to assess the antibacterial and antifungal activity of Morinda reticulata Gamble extracts against antibiotic-resistant pathogenic bacteria and toxin producing moulds, respectively. Morinda reticulata Gamble wassubjected to polyphenolic extraction using different solvents viz., water, Ethanol, Acetone, and Methanol. Antibiotic-resistant strains of Staphylococcus aureus, Enterococcus faecalis, Enterobacter aerogenes, Salmonella typhimurium, and Escherichia coli were screened for the antibacterial activity of different grape extracts. Antibacterial activity was analyzed using the agar well diffusion method. Penicillium chrysogenum, Penicillium expansum, Aspergillus niger and Aspergillus versicolor were screened for antifungal activity. Antifungal activity was determined by counting non-germinated spores in the presence of peel extracts. As compared to other solvent extracts, methanol extracts possessed high antibacterial and antifungal activity. S.typhimurium and E.coli showed complete resistance against antibacterial action at screened concentrations of grape peel extracts. Maximum zone of inhibition was found in case of S.aureus, i.e., 22 mm followed by E.faecalis and E.aerogenes, i.e., 18 and 21 mm, respectively, at 1080 mg Tannic Acid Equivalent (TAE)/ml. The maximum and minimum percent of growth inhibition was shown by P.expansum and A.niger as 73% and 15% at 1080 TAE/ml concentration of grape peel extract, respectively. Except for S.typhimurium and E.coli, the growth of all bacterial and mould species was found to be significantly (P < 0.05) inhibited by all the solvent extracts. [ABSTRACT FROM AUTHOR] |
