Genome editing of a rice CDP-DAG synthase confers multipathogen resistance.

Autor: Sha, Gan, Sun, Peng, Kong, Xiaojing, Han, Xinyu, Sun, Qiping, Fouillen, Laetitia, Zhao, Juan, Li, Yun, Yang, Lei, Wang, Yin, Gong, Qiuwen, Zhou, Yaru, Zhou, Wenqing, Jain, Rashmi, Gao, Jie, Huang, Renliang, Chen, Xiaoyang, Zheng, Lu, Zhang, Wanying, Qin, Ziting
Zdroj: Nature; Jun2023, Vol. 618 Issue 7967, p1017-1023, 7p
Abstrakt: The discovery and application of genome editing introduced a new era of plant breeding by giving researchers efficient tools for the precise engineering of crop genomes1. Here we demonstrate the power of genome editing for engineering broad-spectrum disease resistance in rice (Oryza sativa). We first isolated a lesion mimic mutant (LMM) from a mutagenized rice population. We then demonstrated that a 29-base-pair deletion in a gene we named RESISTANCE TO BLAST1 (RBL1) caused broad-spectrum disease resistance and showed that this mutation caused an approximately 20-fold reduction in yield. RBL1 encodes a cytidine diphosphate diacylglycerol synthase that is required for phospholipid biosynthesis2. Mutation of RBL1 results in reduced levels of phosphatidylinositol and its derivative phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). In rice, PtdIns(4,5)P2 is enriched in cellular structures that are specifically associated with effector secretion and fungal infection, suggesting that it has a role as a disease-susceptibility factor3. By using targeted genome editing, we obtained an allele of RBL1, named RBL1Δ12, which confers broad-spectrum disease resistance but does not decrease yield in a model rice variety, as assessed in small-scale field trials. Our study has demonstrated the benefits of editing an LMM gene, a strategy relevant to diverse LMM genes and crops.Editing of a rice gene that has a role in phospholipid synthesis has endowed rice plants with broad-spectrum resistance to disease, including protection from common bacterial and fungal pathogens, without decreasing the yield. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
Externí odkaz: