| Abstrakt: |
Aimé S Ouédraogo,2,3 Arsène W Nikiema,1,2 Moustapha Abdoulaye Gambo,1,2 Norbert Ramde,2,3 Estelle NH Youl,1,2 Assita Sanou-Lamien,2,3 Olga Mélanie Lompo,2,3 Pierre I Guissou1,21Laboratoire de Développement du Médicament, Ecole Doctorale Sciences et Santé (ED2S), Université Joseph KI-ZERBO, Ouagadougou, Burkina Faso; 2Faculté des Sciences de la Santé, Université Joseph KI-ZERBO, Ouagadougou, Burkina Faso; 3Laboratoire d'Anatomie Pathologique, Centre Hospitalier Universitaire Yalgado Ouédraogo, Ouagadougou, Burkina FasoCorrespondence: Moussa Ouédraogo, Laboratoire de Développement du Médicament, Université Joseph KI-ZERBO, BP: 7021 Ouagadougou 03, Tel +226 25 30 70 64 /65, Email ouem [email protected] Purpose: This study aimed to provide pharmacological evidence of Pseudocedrela kotschyi and Ximenia americana in preventing or healing peptic ulcers claimed by traditional healers in Burkina Faso. Methods: The trunk bark of Pseudocedrela kotschyi and the roots bark of Ximenia americana (Olacaceae) were macerated in mixed ethanol/water (80:20), respectively, to obtain dried extracts. Two models of hydrochloric acid (HCl, 0.3 M/ethanol, 60%) and hypothermic stress-induced peptic ulcer were used. The cytoprotective effect of individual or combined plant extracts was assessed at 1; 10; 30mg/kg. bw. Then, the healing effect of the extracts at 10mg/kg.bw was evaluated within 21 days of treatment on the hydrochloric acid-induced ulcer model. The extracts' antioxidant activity and phenolic content were assessed to support the plant extracts' efficiency. Results: The extracts of P. kotschyi and X. americana at 10 mg/kg.bw reduced ulceration index in hydrochloric acid- and hypothermic stress-ulcer models by more than 83% and 65%, respectively. The extract from X. americana at 10mg/kg.bw allowed complete ulcer healing but not the association of the two plant extracts. The plant extracts had IC50of inhibition of DPPH radical lower than 5μg/mL and total ferric reducing antioxidant power of more than 77 mg EQAA/100mg. The total polyphenolic content was 64.82 ± 0.99 and 53.75 ± 1.39 mg EGA/g of dried extract of P. kotschyi and X. americana, respectively. Conclusion: X. americana extract is better than the combined two plant extracts in gastric cytoprotection and ulcer healing. Further investigations are needed to highlight mechanism-based effects. [ABSTRACT FROM AUTHOR] |
