| Autor: |
Greenlee, Justin J, Moore, S Jo, Cassmann, Eric D, Lambert, Zoe J, Kokemuller, Robyn D, Smith, Jodi D, Kunkle, Robert A, Kong, Qingzhong, Greenlee, M Heather West |
| Předmět: |
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| Zdroj: |
Journal of Infectious Diseases; Jun2023, Vol. 227 Issue 12, p1386-1395, 10p |
| Abstrakt: |
Background: Classical scrapie is a prion disease of sheep and goats that is associated with accumulation of abnormal prion protein (PrPSc) in the central nervous and lymphoid tissues. Chronic wasting disease (CWD) is the prion disease of cervids. This study was conducted to determine the susceptibility of white-tailed deer (WTD) to the classical scrapie agent.Methods: We inoculated WTD (n = 5) by a concurrent oral/intranasal exposure with the classical scrapie agent from sheep or oronasally with the classical scrapie agent from goats (n = 6).Results: All deer exposed to the agent of classical scrapie from sheep accumulated PrPSc. PrPSc was detected in lymphoid tissues at preclinical time points, and deer necropsied after 28 months post-inoculation had clinical signs, spongiform lesions, and widespread PrPSc in neural and lymphoid tissues. Western blots on samples from the brainstem, cerebellum, and lymph nodes of scrapie-infected WTD have a molecular profile similar to CWD and distinct from samples from the cerebral cortex, retina, or the original classical scrapie inoculum. There was no evidence of PrPSc in any of the WTD inoculated with classical scrapie prions from goats.Conclusions: WTD are susceptible to the agent of classical scrapie from sheep and differentiation from CWD may be difficult. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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