| Autor: |
Valojerdi, Fereshte Mahdizade, Goliaei, Bahram, Rezakhani, Nakisa, Nikoofar, Alireza, Neghab, Hoda Keshmiri, Soheilifar, Mohammad Hasan, Bigdeli, Bahareh |
| Předmět: |
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| Zdroj: |
Middle East Journal of Cancer; Apr2023, Vol. 14 Issue 2, p205-218, 14p |
| Abstrakt: |
Background: Radiotherapy is a frequently used therapeutic modality for breast cancer. Dalbergin, a natural antioxidant, inhibits carcinogens and tumor progression. In the present study, we investigated the effect of Dalbergin on the response of T47D and MDA-MB-231 breast cancer cell lines to ionizing radiation. Method: In this experimental in vitro study, doubling time of T47D and MDA-MB-231 were obtained from the growth curve. The cytotoxic effect of Dalbergin on T47D and MDA-MB-231 breast cancer cells were estimated via MTT assay. To determine the clonogenic ability, we treated T47D and MDA-MB-231 with Dalbergin for 48 h prior to irradiation, subsequent to which a colony assay was performed. Realtime polymerase chain reaction was employed to determine the gene expression level. Results: Dalbergin inhibited proliferation of T47D and MDA-MB-231 in a time-and concentration-dependent manner. Additionally, the most appropriate time for the treatment of these types of cancer cells was found to be 48 h and the drug's concentration in both cell lines was different. The IC50 values of T47D and MDA-MB-231 cells were 0.001 and 0.0001 μM, respectively. Moreover, this drug radiaosensitizes both cell lines effectively compared with the radiation only. Finally, the gene expression level of p53, Bcl-2, and STAT3 were investigated in cancer cells. Conclusion: Dalbergin showed apoptotic effects probably through the STAT/p53 signaling pathway. Therefore, Dalbergin could be considered as a radiosensitizer and its effects may be owing to increased cell death. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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