| Autor: |
Porzucek, Abigail J, Proctor, Abigale M, Klinkhammer, Katharina E, Tritsch, Sarah R, Robertson, Molly A, Bashor, Jonathan P, Villani, Jack, Sepulveda, Jorge L, Mores, Christopher N |
| Předmět: |
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| Zdroj: |
Journal of Infectious Diseases; May2023, Vol. 227 Issue 9, p1084-1087, 4p |
| Abstrakt: |
During the 2022 monkeypox (MPX) outbreak, testing has been limited and results delayed, allowing ongoing transmission. Gold-standard quantitative polymerase chain reaction (qPCR) diagnostics are difficult to obtain. This research adapted the June 2022 CDC MPX qPCR assay for broad implementation. Validated using MPX stocks in a matrix with multiple sample types, MPX was detected with cycle threshold (Ct) values 17.46–35.59 and titer equivalents 8.01 × 106 to 2.45 × 100 plaque–forming unit (PFU)/mL. The detection limit was 3.59 PFU/mL. Sensitivity and specificity were both 100%. This qPCR assay can be quickly and broadly implemented in research and public health laboratories to increase diagnostic capacity amid the growing MPX outbreak. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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