| Abstrakt: |
To analyze the treatment effect of the curcumin nanoparticle drug delivery system to the Alzheimer's disease (AD), the NP-Cur, NP-S1-Cur, and NP-S1-Cur-CRT nanoparticles were prepared by combining the various internal water phases based on the emulsification volatilization method for the cytotoxicity test and applied to treatment of β-amyloid precursor protein/pistilloid-stamen 1 (APP/PS1) double transgenic (DT) mice in three groups, which were group W1 (treated with NP-Cur), W2 (treated with NP-S1-Cur), W3 (treated with NP-S1-Cur-CRT), W4 (treated with curcumin drugs), and W0 (normal control), respectively. Immunohistochemistry was adopted to detect the senile plaques (amyloid precursor antibody, 6E10), microglia (IBA-1), and astrocytes (glial fibrillary acidic protein, GFAP). The spatial memory ability (SMA) of mice was evaluated by the Y maze experiment, and the learning memory ability (LMA) of mice was evaluated by the new thing cognition experiment. The results showed that in the human neuroblastoma cell (SH-SY5Y), mouse brain microvascular endothelial cell line (bEnd.3), and mouse microglia cell line (BV2), the cell activity (CA) caused by NP-Cur, NP-S1-Cur, and NP-S1-Cur-CRT nanoparticles all decreased with the increase of concentration value of curcumin, but all were higher than 80%; it was found based on the Y maze experiment that the stay time (ST) and the number of entries (NE) in the group W4 were higher than those in group W1, W2, and W3 (P < 0.05); the frequency of exploring new things in the group W0 was higher than the frequency in W1, W2, and W3 (P < 0.05). There was no remarkable difference in the exploration frequency of new things in group W4 and W0 (P > 0.05); the positive units (PU values) of cytoplasm and nucleus (C&N) 6E10 protein in W4 were lower than the values in W1, W2, and W3 (P > 0.05); the PU value of C&N IBA-1 protein of W4 was lower than the value in W1, W2, and W3 (P < 0.05); the PU value of C&N GFAP protein expression in group W4 was lower obviously than the value in W1, W2, and W3 (P < 0.05). In short, poly lactic-co-glycolic acid (PLGA) nanoparticles loaded with curcumin had good biosafety and compatibility. It can effectively improve the LMA of APP/PS1 mice, reduce the area of senile plaques, and have better neuroprotective function. Among them, the NP-S1-Cur-CRT nanoparticles had the best improvement effect. [ABSTRACT FROM AUTHOR] |
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