| Autor: |
Ikegawa, Yuko, Combet, Christophe, Groussin, Mathieu, Navratil, Vincent, Safar‐Remali, Sabrina, Shiota, Takuya, Aouacheria, Abdel, Yoo, Sa Kan |
| Předmět: |
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| Zdroj: |
EMBO Journal; 4/17/2023, Vol. 42 Issue 8, p1-14, 14p |
| Abstrakt: |
Cells need to sense stresses to initiate the execution of the dormant cell death program. Since the discovery of the first BH3‐only protein Bad, BH3‐only proteins have been recognized as indispensable stress sensors that induce apoptosis. BH3‐only proteins have so far not been identified in Drosophila despite their importance in other organisms. Here, we identify the first Drosophila BH3‐only protein and name it sayonara. Sayonara induces apoptosis in a BH3 motif‐dependent manner and interacts genetically and biochemically with the BCL‐2 homologous proteins, Buffy and Debcl. There is a positive feedback loop between Sayonara‐mediated caspase activation and autophagy. The BH3 motif of sayonara phylogenetically appeared at the time of the ancestral gene duplication that led to the formation of Buffy and Debcl in the dipteran lineage. To our knowledge, this is the first identification of a bona fide BH3‐only protein in Drosophila, thus providing a unique example of how cell death mechanisms can evolve both through time and across taxa. Synopsis: BH3‐only proteins act as important stress sensors that induce apoptosis. In spite of their central role in other species, BH3‐only proteins have not been found in Drosophila. The current study identifies the first Drosophila BH3‐only protein, sayonara, that induces apoptosis in a BH3 motif‐dependent manner and interacts with Drosophila BCL‐2 homologs. In silico analysis identifies the Drosophila BH3‐only protein sayonara.Sayonara induces apoptosis in a BH3 motif‐dependent manner.Sayonara interacts genetically and biochemically with the BCL‐2 proteins, Buffy and Debcl.There is a feedback loop between Sayonara‐mediated caspase activation and autophagy. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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