| Autor: |
Kubitschke, Martin, Müller, Monika, Wallhorn, Lutz, Pulin, Mauro, Mittag, Manuel, Pollok, Stefan, Ziebarth, Tim, Bremshey, Svenja, Gerdey, Jill, Claussen, Kristin Carolin, Renken, Kim, Groß, Juliana, Gneiße, Pascal, Meyer, Niklas, Wiegert, J. Simon, Reiner, Andreas, Fuhrmann, Martin, Masseck, Olivia Andrea |
| Předmět: |
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| Zdroj: |
Nature Communications; 12/6/2022, Vol. 13 Issue 1, p1-17, 17p |
| Abstrakt: |
We developed a family of genetically encoded serotonin (5-HT) sensors (sDarken) on the basis of the native 5-HT1A receptor and circularly permuted GFP. sDarken 5-HT sensors are bright in the unbound state and diminish their fluorescence upon binding of 5-HT. Sensor variants with different affinities for serotonin were engineered to increase the versatility in imaging of serotonin dynamics. Experiments in vitro and in vivo showed the feasibility of imaging serotonin dynamics with high temporal and spatial resolution. As demonstrated here, the designed sensors show excellent membrane expression, have high specificity and a superior signal-to-noise ratio, detect the endogenous release of serotonin and are suitable for two-photon in vivo imaging. Genetically encoded sensors have been developed and become versatile tools for imaging serotonin dynamics. Here, authors developed a family of serotonin (5-HT) sensors (sDarken), three variants with different affinities for 5-HT enable high spatiotemporal resolution of 5-HT dynamics. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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