Effects of freeze-thaw cycles on physicochemical properties and structure of cooked crayfish (Procambarus clarkii).

Autor: Han, Jiping, Sun, Yingjie, Sun, Rongxue, Zhang, Tao, Wang, Cheng, Jiang, Ning
Předmět:
Zdroj: Food Production, Processing & Nutrition; 10/5/2022, Vol. 4 Issue 1, p1-14, 14p
Abstrakt: To explore the damage mechanisms of freeze-thaw cycles on cooked crayfish in frozen storage, changes in the physicochemical properties and structure of cooked crayfish during the freeze-thaw cycles were investigated. The physicochemical properties of cooked crayfish changed significantly after five freeze-thaw cycles. The moisture content, water holding capacity, pH, and textural properties were decreased, while the total color difference, drip loss, and protein and lipid oxidation were significantly increased (P < 0.05). LF-NMR and MRI verified the water loss, and SDS-PAGE showed denaturation/degradation of myofibrillar proteins (MPs). Multiple freeze-thaw cycles promoted the transition from α-helix to β-turn in the secondary structure, the unfolding of tertiary structure, and a significant change in the chemical forces of MPs. SEM results revealed a disruption in the microstructure of muscle fibers. Repeated freeze-thaw cycles reduced the moisture content and distorted the structure of MPs in cooked crayfish, resulting in the disruption of physicochemical properties and its structure. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index