| Autor: |
Déziel, Eric, Gopalan, Suresh, Tampakaki, Anastasia P., Lépine, François, Padfield, Katie E., Saucier, Maude, Xiao, Gaoping, Rahme, Laurence G. |
| Předmět: |
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| Zdroj: |
Molecular Microbiology; Feb2005, Vol. 55 Issue 4, p998-1014, 17p |
| Abstrakt: |
The transcriptional regulator MvfR is required for fullPseudomonas aeruginosavirulence, the function of multiple quorum sensing (QS)-regulated virulence factors and the synthesis of 4-hydroxy-2-alkylquinolines (HAQs), including thePseudomonasquinolone signal (PQS). Here we investigate the role of MvfR in the QS circuitry andP. aeruginosapathogenesis. We demonstrate using a combination of biochemical and molecular approaches, including transcription profiling, that MvfR is involved in the regulation of multipleP. aeruginosaQS-controlled genes without altering the expression oflasRI/ rhlRIor the production ofN-acyl-l-homoserine lactone (AHL) signals. Dissection of howmvfRis interwoven into theP. aeruginosaQS circuitry reveals that the MvfR system, through the essential contribution of PqsE, positively regulates a subset of genes dependant on both LasR and RhlR. Animal studies show that MvfR contributes toP. aeruginosavirulence by controlling the transcription of genes not under RhlR regulation, and that reduced virulence of amvfRmutant is caused by the loss ofpqsEexpression and not only a deficiency in HAQs/PQS production. This study provides novel insights into the unique role of the MvfR system in AHL-mediated QS and further supports its importance inP. aeruginosapathogenesis. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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