Long‐Term Observation of Locomotion of Drosophila Larvae Facilitates Feasibility of Food‐Choice Assays.

Autor:	Bittern, Jonas, Praetz, Marit, Baldenius, Marie, Klämbt, Christian
Předmět:	ANIMAL locomotion DROSOPHILA ANIMAL behavior LARVAE IMAGE analysis NEURAL circuitry FOOD preferences
Zdroj:	Advanced Biology; Apr2022, Vol. 6 Issue 4, p1-13, 13p
Abstrakt:	Animal behavior is reflected by locomotor patterns. To decipher the underlying neural circuitry locomotion has to be monitored over often longer time periods. Here a simple adaptation is described to constrain movement of third instar Drosophila larvae to a defined area and use Frustrated total internal reflection based imaging method (FIM) imaging to monitor larval movements up to 1 h. It is demonstrated that the combination of FIM imaging and long analysis periods facilitates the conduction of food choice assays and provides the means to easily quantify food preferences. [ABSTRACT FROM AUTHOR]
Databáze:	Complementary Index
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