Supramolecular interactions mediated thermal stabilization for α-amylase modified with a β-cyclodextrin-carboxymethylcellulose polymer.

Autor: Darias, Rodolfo, Herrera, Ivelises, Fragoso, Alex, Cao, Roberto, Villalonga, Reynaldo
Předmět:
Zdroj: Biotechnology Letters; Oct2002, Vol. 24 Issue 20, p1665-1668, 4p
Abstrakt: α-Amylase from Bacillus subtilis was modified with a β-cyclodextrin-carboxymethylcellulose polymer and retained 90% of its initial activity. Its thermostability was enhanced from 68 °C to 82.5 °C over 10 min incubation and the resistance to inactivation at 75 °C was increased 5-fold. The influence of supramolecular associations polymer-protein on enzyme thermostabilization was demonstrated. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index