| Autor: |
Engibarov, Stephan, Tsekovska, Rositsa, Kirilov, Kiril, Gatev, Evan, Ivanov, Ivan, Mironova, Roumyana, Handzhyiski, Yordan |
| Předmět: |
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| Zdroj: |
Journal of Chemical Technology & Metallurgy; 2022, Vol. 57 Issue 2, p291-297, 7p |
| Abstrakt: |
Non-enzymatic cleavage of nucleic acids (DNA or RNA) is essential for developing novel antibiotics and chemotherapeutics, as well as new gene manipulation techniques. In this study, we investigated for deoxyribonuclease activity the allenic acids 2-methyl-4-phenylhexa-2,3-dienoic acid (racemic mixture, AK-1), S-2-methyl-4-phenylbuta-2,3-dienoic acid (AK-2) and R-2-methyl-4-phenylbuta-2,3-dienoic acid (AK-3) using supercoiled plasmid pUC18 DNA as a substrate. All three allenic acids demonstrated single strand deoxyribonuclease activity in the order AK2 = AK3 > AK1. Increasing concentrations of potassium-phosphate buffer (pH 7.5) in the reaction mixture completely inhibited the relaxation of the supercoiled pUC18 DNA, thus implying that the phosphodiester bond in DNA is the vulnerable site of attack by the allenic acids. When plasmid pUC18 DNA nicked with allenic acids was treated with T4 DNA ligase, it failed to regain its covalently closed circular form. This indicates that the allenic acids act as either hydrolytic agents producing 3'-PO4 and 5'-OH termini, or oxidizers, damaging DNA by free radical mechanism. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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