| Autor: |
OSMAN, AHMED L., ALHATTI, REEM, SAWAS, HIBA, ALTOUM, ABD ELGADIR A., SHADROCH, DEVAPRIYA FINNEY, EL-SAYED, WALID, KANDAKURTIC, PRAVEEN KUMAR, BABKER, ASAAD M. A. |
| Předmět: |
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| Zdroj: |
International Journal of Pharmaceutical Research (09752366); Jul-Sep2020, Vol. 12 Issue 3, p2587-2593, 7p |
| Abstrakt: |
Aim To evaluate the bactericidal effects of ozone Nano-bubble water (NBW3) in different pathogenic bacteria. Methodology 0.1 mL volume of bacteria suspension containing approximately 1 x 107 CFUs/ mL (McFarland standard) of each test bacterium added to each 10 mL of serial dilution of ozone nanobubbles water, 0.9% NaCl with standard strains of bacteria (as negative control) used. Following the incubation (for 2, 5, and 10 min, at 37 °C). The 0.9% NaCl control bacterial suspensions incubated and treated in the same manner. Aliquots removed from each tube at each time point and serial 10-fold dilutions prepared in sterile physiological saline. Next, 0.1 mL aliquots of each dilution aseptically inoculated on plate of agar medium and incubated at 37 °C for overnight. Colonies then be counted, and the number of CFUs/mL be plotted against time for each bacterium tested. Results Ozone had an antibacterial effect on both gram positive and gram-negative bacteria those suspended in first 2 delusion, but little effect in third one. Its antibacterial efficacy was not comparable with that of NaCl under the test conditions used. Ozone-Nano effect on gram negative and gram-positive bacteria. Conclusion Ozone-Nano-bubbles water has bactericidal effect on different strains of gram negative and gram-positive bacteria. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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