Benefit of coupling heparin to crosslinked collagen I/III scaffolds for human dermal fibroblast subpopulations' tissue growth.

Autor: Michopoulou, Anna, Koliakou, Eleni, Terzopoulou, Zoi, Rousselle, Patricia, Palamidi, Artemis, Anestakis, Doxakis, Konstantinidou, Polyanthi, Roig‐Rosello, Eva, Demiri, Euterpi, Bikiaris, Dimitris
Zdroj: Journal of Biomedical Materials Research, Part A; Apr2022, Vol. 110 Issue 4, p797-811, 15p
Abstrakt: Currently, there is a lack of models representing the skin dermal heterogeneity for relevant research and skin engineering applications. This is the first study reporting production of dermal equivalents reproducing features of papillary and reticular dermal compartments. Inspired from our current knowledge on the architecture and composition differences between the papillary and reticular dermis, we evaluated different collagen‐based porous materials to serve as scaffolds for the three‐dimensional expansion of freshly isolated papillary and/or reticular fibroblasts. The scaffolds, composed of either collagen I or collagen I and III mixtures, were prepared by lyophilization. Pore size and hydrolytic stability were controlled by crosslinking with 1‐ethyl‐3‐(3‐dimethyl aminopropyl) carbodiimide (EDC) and N‐hydroxysuccinimide (NHS) or EDC/NHS with covalently bound heparin. The evaluation of the resultant "papillary" and "reticular" dermal equivalents was based on the analysis of characteristic features of each dermal compartment, such as cell density and deposition of newly synthetized extracellular matrix components in histological sections. Crosslinking supported cell growth during dermal tissue formation independent on the fibroblast subpopulation. The presence of collagen III seemed to have some positive but non‐specific effect only on the maintenance of the mechanical strength of the scaffolds during dermal formation. Histological analyses demonstrated a significant and specific effect of heparin on generating dermal equivalents reproducing the respective higher papillary than reticular cell densities and supporting distinct extracellular matrix components deposition (three to five times more carbohydrate material deposited by papillary fibroblasts in all scaffolds containing heparin, while higher collagen production was observed only in the presence of heparin). [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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