| Autor: |
Campos, Ivan T.N., Tanaka-Azevedo, Anita M., Tanaka, Aparecida S. |
| Předmět: |
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| Zdroj: |
FEBS Letters; Nov2004, Vol. 577 Issue 3, p512-516, 5p |
| Abstrakt: |
Recently, we have cloned several Kazal-type serine protease inhibitors from the midgut of the Triatoma infestans bug. A single gene composed of multi Kazal-type domains, in tandem, encodes these inhibitors. In this work, we describe the purification and characterization of recombinant infestins 3-4 and 4, which are potent factor XIIa inhibitors (Ki=67 pM and 128 pM, respectively). We also identified the first native factor XIIa inhibitor from a hematophagous insect. The factor XIIa inhibitory activity of infestin 4 demonstrates extremely efficient anticoagulant activity, prolonging activated partial thromboplastin time by approximately 3 times. Our results suggest that infestins perform a very important role in the T. infestans midgut during meal acquisition and digestion by controlling blood coagulation by means of inhibiting thrombin and factor XIIa. [Copyright &y& Elsevier] |
| Databáze: |
Complementary Index |
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