| Autor: |
Frye, Katie A., Piamthai, Varadh, Hsiao, Ansel, Degnan, Patrick H. |
| Zdroj: |
Cell Reports; Dec2021, Vol. 37 Issue 13, pN.PAG-N.PAG, 1p |
| Abstrakt: |
The functional and genomic diversity of the human gut microbiome is shaped by horizontal transfer of mobile genetic elements (MGEs). Characterized MGEs can encode genes beneficial for their host's self-defense (e.g., antibiotic resistance) or ability to compete for essential or limited resources (e.g., vitamins). Vitamin B 12 and related compounds (corrinoids) are critical nutrients that enable colonization by members of the common gut microbe phylum, the Bacteroidetes. Herein, we identify a distinct class of MGEs in the Bacteroidetes responsible for the mobilization and exchange of the genes required for transport of corrinoids, a group of cyclic tetrapyrrole cofactors including vitamin B 12 (btuGBFCD). This class includes two distinct groups of conjugative transposons (CTns) and one group of phage. Conjugative transfer and vitamin B 12 transport activity of two of the CTns were confirmed in vitro and in vivo , demonstrating the important role MGEs play in distribution of corrinoid transporters in the Bacteroidetes. [Display omitted] • Diverse mobile genetic elements in gut microbes encode vitamin B 12 transporters • Transfer of CTns with B 12 transporters complement transport-deficient strains • CTns with B 12 transporters offer competitive advantages during in vitro growth • In vivo conjugation restores ability to use B 12 in transport-deficient Bacteroides Mobile DNA can improve the competitive abilities of microbes. Frye et al. identify three distinct families of mobile DNA that encode vitamin B 12 transporters in human gut Bacteroidetes. In vitro and in vivo transfer experiments demonstrate the importance of HGT in shaping competition for essential small molecules like B 12. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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