Integrated device for colorimetric detection of arsenite using polyethylene glycol capped gold nanoparticles—Lab-on-chip.

Autor: Shalvi, Kumar, Nitesh, Verma, Kanak Lata, Jain, Vinod Kumar, Nagpal, Suman
Zdroj: Toxicology & Environmental Health Sciences; Dec2021, Vol. 13 Issue 4, p351-362, 12p
Abstrakt: Objective: The importance of the detection of As (III) heavy metal has been increased due to its toxicity in the environment, which is ultimately affecting all types of organisms depending upon the water which is the prime source of life. Application of the colorimetric probe applied to tissue samples because As (III) is the kind of heavy metal that can remain in the body for a prolonged time and can be retrieved even from the cadaver as it acts as a slow poison. Therefore, it is essential to detect its presence from a biological sample like viscera (i.e. tissues) in a user-friendly and cost-effective way without compromising the detection sensitivity and selectivity. Hence, in this work, we present a novel approach towards the detection of As (III) in tissue samples using an integrated hand-held device enabling high sensitivity, selectivity, and reproducibility of the detection. Methods: The present study, involved the preparation and characterization of polyethylene capped gold nanoparticles (PEG-AuNPs) nanocomposite using various characterization tools. An electrostatic interaction causes the aggregation of As (III) on binding with the nanocomposites, which can be detected colorimetrically. Further, the work involved the in-house fabrication of an integrated hand-held device for on-the-spot quantification of the As (III) ions complex in tissue samples with high selectivity and sensitivity. The results were compared and correlated with known standard methods. Results: The PEG-AuNPs exhibit a specific colour change with respect to As (III) in both the platforms (water and tissue samples) after interacting with various concentrations of As (III) in solution. The sensing capability of our hand-held device using PEG-AuNPs showed a good correlation (r = 0.98) with known standard methods, i.e. UV–Vis spectroscopy and ICP–OES, under optimized conditions. Conclusion: PEG-AuNPs are highly sensitive and selective for As (III) even in the presence of various interfering agents and the detection limit of our device for tissue samples was found to be 2.9 ppm. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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