Comparison in Synergetic Effect for Silver Nanoparticles that Produced from P.aeruginosa and P.luteola with Oil Extract of Cymbopogon Citraus on Different Types of Pathogenic Bacteria.

Autor: Ahmed, Suaad Ali, Hasan, Hussam Mahmood
Předmět:
Zdroj: Indian Journal of Forensic Medicine & Toxicology; Oct-Dec2021, Vol. 15 Issue 4, p2589-2595, 7p
Abstrakt: Silver nanoparticles have been found to possess both anti-bacterial and anti-inflammatory properties, In addition Many reports refered to the antibacterial activity of lemongrass oil against different organisms. Two species of Gram negative bacteria Psuedomonas (P.auroginosa and P.luteola) were tested for the ability of bearing silver nanoparticles by using LB medium, the two isolates of bacteria were appeared brown color when mixed the supernatant of bacterial culture with AgNO3 solution, that referred the biosynthesis of Silver nanoparticles (Ag NPs). UV-visible spectrophotometer was utilized for estimation of (Ag NPs). The antimicrobial activity of silver nanoparticles arranged from the two isolates was tested against different types of pathogenic bacteria. lemongrass oil was extracted and three different concentrations (v/v) of lemongrass oil with dimethyl sulfoxide (DMSO) 0.5%, 5% and 10%, were prepared to determin the MIC by using well diffution method. The synergetic effect was demonstrated by combining the (Ag NPs) that produced from, Pseudomonas aeruginosa and Pseudomonas luteola with MIC of lemongrass oil (0.5%) against same types of clinical bacteria. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index