| Autor: |
Yojiro Minegishi, Katsufumi Uchiyama, Keizo Sakurai, Shiro Ibe, Hiromi Kanda, Shin Nihonyanagi, Masaki Nakamura, Shinsuke Ikeda, Masashi Takaso |
| Předmět: |
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| Zdroj: |
Modern Rheumatology; 2019, Vol. 29 Issue 5, p867-873, 7p |
| Abstrakt: |
Objectives: Polymerase chain reaction (PCR)-based assays are being increasingly used for the diagnosis of orthopedic-related infections. Unfortunately, classical PCR requires imaging devices that are expensive and complex. We previously developed the PCR-lateral flow (PCR-LF) method, which does not require any additional imaging device. In the present study, the objective was to determine whether PCR-LF tests could be used to effectively diagnose orthopedic-related infections. Methods: In this study, we used PCR-LF to diagnose common causes of orthopedic-related infections and compared the results to those from conventional bacterial cultures of the same samples. Results: Notably, for 228 synovial fluid or pus specimens, the sensitivity and specificity of bacterial cultures were 53.5% and 97.7%, respectively, compared to 61.6% and 89.9% for PCR-LF. Although the difference in sensitivity between bacterial cultures and PCR-LF was not significant, when our analysis was limited to cases with suspected periprosthetic joint infection, the sensitivity of PCR-LF (66.1%) was superior to that of bacterial cultures (42.9%). Conclusion: This study indicates that PCR-LF is a useful method for diagnosing orthopedicrelated infections. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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