Effectiveness of microscopy (using 10-20% KOH with or without lacto-phenol cott on blue) in identifying fungal elements in diabetic foot ulcer.

Autor: Khalid, Muhammad, Sabir, Muhammad, Bashir, Shahida, Ahmed, Raziuddin, Indhar, Fakharuddin
Předmět:
Zdroj: Pakistan Journal of Surgery; Apr-Jun2021, Vol. 37 Issue 2, p112-115, 4p
Abstrakt: Objective: Chronic, uncontrolled diabetes mellitis is associated with dysfunctions of the immune and healing systems. Consequently, repeated infections by bacteria and/or fungi and the sluggish healing process, pose a constant threat to the integrity of tissues, particularly the frequently injuring feet, leading to grave complications. This study assesses the identification of fungal elements in scrapings from diabetic foot ulcer through direct microscopy using 10% potassium hydroxide and lacto-phenol cott on blue. Material and Methods: In this study, we included 298 diabetic patients with foot ulcers from June 2016 to June 2019. We recorded their demographic features, blood glucose at the time of clinical examination, and the grades of the ulcers according to Wagner ulcer grading scale. The samples from scrapings were taken with sterilized instruments from the edges of the ulcers and handled as per recommended guidelines. Results: Fungal positivity was more in men (56.7%) than women (43.3%). Overall mean (±SD) age was 50.0 (± 8.1). On fungal culture, total fungus positive cases were 30.9%. On simple microscopy, using KOH/LPCB, dermatophytes were identified in 95% (p < 0.05) of the true positive cases. Conclusion: The microscopy of skin scrapings from diabetic foot ulcer, using 10-20% potassium hydroxide and lacto-phenol cott on blue, is a simple and effective out-patient technique with an acceptable yield in terms of identifying fungal elements. Other similar studies have shown corresponding conclusions. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index