| Abstrakt: |
In this study, a total of 12 genotypes were used, including 1 hulled emmer and 11 registered ones which are important as genetic resources and for durum wheat cultivation. The responses of these genotypes to different drought stress levels were determined in vitro. In this research, to create drought stress, high molecular weight polyethylene glycol (PEG 6000) was used. In the trials, 5 different PEG 6000 doses were administered to induce drought stress with rates of 0 (control), -0.50 bar, -1.48 bar, -2.95 bar and -4.91 bar. During the experiments, after the callus was formed with the endosperm supported mature embryo culture, regeneration capacity and plantlets they formed were evaluated by applying different levels of drought stress to the callus. Using these parameters, stress tolerance index, stress sensitivity index and tolerance index values of durum wheat genotypes were calculated. According to the results, there was a significant decrease in all parameters examined with the increase of drought stress. While Artuklu and Sarıçanak-98 cultivars had the highest drought tolerance, Çakmak-79, ÇeÅŸit-1252, Eminbey and Kunduru-1149 cultivars were sensitive to drought and Triticum dicoccum, which is an important gene source, was also sensitive. In this study, a total of 12 genotypes were used, including 1 hulled emmer and 11 registered ones which are important as genetic resources and for durum wheat cultivation. The responses of these genotypes to different drought stress levels were determined in vitro. In this research, to create drought stress, high molecular weight polyethylene glycol (PEG 6000) was used. In the trials, 5 different PEG 6000 doses were administered to induce drought stress with rates of 0 (control), -0.50 bar, -1.48 bar, -2.95 bar and -4.91 bar. During the experiments, after the callus was formed with the endosperm supported mature embryo culture, regeneration capacity and plantlets they formed were evaluated by applying different levels of drought stress to the callus. Using these parameters, stress tolerance index, stress sensitivity index and tolerance index values of durum wheat genotypes were calculated. According to the results, there was a significant decrease in all parameters examined with the increase of drought stress. While Artuklu and Sarıçanak-98 cultivars had the highest drought tolerance, Çakmak-79, Çeşit-1252, Eminbey and Kunduru-1149 cultivars were sensitive to drought and Triticum dicoccum, which is an important gene source, was also sensitive. [ABSTRACT FROM AUTHOR] |
