| Autor: |
Chen, Suting, Teng, Tianlu, Zhang, Zhuman, Shang, Yuanyuan, Xiao, Hua, Jiang, Guanglu, Wang, Fen, Jia, Junnan, Dong, Lingling, Zhao, Liping, Chu, Naihui, Huang, Hairong |
| Předmět: |
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| Zdroj: |
Infection & Drug Resistance; Mar2021, Vol. 14, p1199-1208, 10p |
| Abstrakt: |
aim of this study was to determine the inhibitory activity of CCCP as a potential novel antibiotic against M. abscessus. Methods: A total of 47 reference strains of different mycobacterial species and 60 clinical isolates of M. abscessus were enrolled. In vitro inhibitory activity of CCCP was accessed using microplates alamar blue method with the reference and clinical isolates. The activity of CCCP against intracellular M. abscessus residing within macrophage was also evaluated by intracellular colony numerating assay. Results: CCCP exhibited good activity against M. abscessus clinical isolates in vitro, the minimum inhibitory concentration (MIC) ranged from 0.47 μg/mL to 3.75 μg/mL, with a MIC50 of 1.875 μg/mL and MIC90 of 3.75 μg/mL. At concentrations safe for the cells, CCCP exhibited highly intracellular bactericidal activities against M. abscessus and M. massiliense reference strains, with inhibitory rates of 84.8%± 8.8% and 72.5%± 13.7%, respectively. CCCP demonstrated bactericidal activity against intracellular M. abscessus that was comparable to clarithromycin, and concentration-dependent antimicrobial activity against M. abscessus in macrophages was observed. In addition, CCCP also exhibited good activities against most reference strains of rapidly growing mycobacterial species. Conclusion: CCCP could be a potential candidate of novel antimicrobiological agent to treat M. abscessus infection. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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