| Autor: |
Shanshan LIANG, Mingjie FAN, Weijian LIANG, Huanying PANG, Chuanhao PAN, Dawei SONG, Mingsheng QIU, Jichang JlAN |
| Předmět: |
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| Zdroj: |
Agricultural Biotechnology (2164-4993); Dec2018, Vol. 7 Issue 6, p1-8, 6p |
| Abstrakt: |
In this study' Hy322 gene was cloned from Vibrio alginolyticus. The total length of its gene was 969 bp' and it could encode 322 amino acids. The physicochemical properties, protein structure, genetic evolutionary relationship and antigenic characteristics of the effector protein Hy322 of V/ alginolyticus HY9901 type III secretion systemwere studied and analyzed by bioinformatics methods and tools. The results showed that Hy322 is an unstable hydrophilic and acidic protein without a transmembrane region and a signal pepide' and secondary sUucture to oí-helix. The evolutionary analysis showed that V alginolyticus HY9901 and V. harveyi were clustered together' which indicated that the genetic relationship between tie two species was closest. HY322 contains a FliN ssper family conserved domain associated with Flagellar motor switch. Bioinformatics analysis sZowed that the B-cell preponderant epitooes of Hy322 might be localized in the regions of 32-33' 100 -102' 138 - 140' 215 -216' 235 - 238 and 246 - 249. The 3D structure model of Hy322 ssbunit was simulated by SWISS-MODEL software and itwas found that the yscQof Yersinia were similar and the similarity was 42. 25 %. In this study' the feasibility of Hy322 as a common antigen of Virio was veūfied from the perspective of bioinformatics' which laid the foundation for the next step in vaccine development. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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