Saliva pools for screening of human cytomegalovirus using real-time PCR.

Autor: Fernandes, Cláudia, Marques, Augusta, de Jesus Chasqueira, Maria, Braz, Mónica Cró, Ferreira, Ana Rute, Neto, Ana Serrão, Mendes, Cândida, Lito, David, Menezes, Maria-Favila, Sousa, Maria José, Paixão, Paulo
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Zdroj: European Journal of Pediatrics; Apr2021, Vol. 180 Issue 4, p1067-1072, 6p, 1 Diagram, 2 Charts
Abstrakt: Human cytomegalovirus (HCMV) is the leading congenital infection agent in the world. The importance of screening this infection has been debated, as 10–15% of the asymptomatic newborns with HCMV at birth will present late sequelae. The aim of this study was to test the feasibility of using saliva pools from newborns in a screening program for congenital HCMV infection, in two Portuguese hospitals. The screening was based on the use of pools of 10 saliva samples for detection of viral DNA by real-time PCR. Whenever there was a positive pool, the samples were tested individually, and for each positive sample the result was confirmed with a urine sample collected in the first 2 weeks of life. The study involved 1492 newborns. One hundred and fifty pools were screened, with 14 positive results in saliva, but only 10 were confirmed in urine samples, giving a prevalence of congenital HCMV infection in both hospitals of 0.67% (CI95% 0.36 to 1.23%). Conclusion: The overall prevalence of congenital HCMV infection in both hospitals was 0.67%. The use of saliva pools proved to be effective for the screening of this congenital infection, allowing timely screening and confirmation in a large population, with associated cost reduction. What is Known: • Newborn screening for HCMV is desirable. • Saliva is a good and practical sample. What is New: • The feasibility of using saliva pools for a large-scale screening. • The cost reduction of this strategy. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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