| Autor: |
MASAMICHI NAKATA, MICHIKO SHIMADA, IKUYO NARITA-KINJO, DAIKI NAGAWA, KAZUTAKA KITAYAMA, MISATO HAMADATE, NAOTAKE MIURA, MASASHI NOZAKA, YOSUKE KAWAMURA, TAKESHI FUJITA, REIICHI MURAKAMI, TADAATSU IMAIZUMI, NORIO NAKAMURA, HIROFUMI TOMITA |
| Předmět: |
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| Zdroj: |
In Vivo; Jan/Feb2021, Vol. 35 Issue 1, p147-153, 7p |
| Abstrakt: |
Background/Aim: Viral infection often exacerbates proteinuria, which has been suggested to be due to antiviral responses of podocytes. We examined the effect of polyinosinic-polycytidylic acid (polyIC) on the expression of retinoic acid-inducible gene-I (RIG-I) and melanoma differentiation-associated gene 5 (MDA5) in differentiated human podocytes in culture. Materials and Methods: The podocytes were treated with 2 ng/ml to 500 μg/ml of polyIC for 3 to 36 h, and also transfected with siRNA against RIGI and MDA5. F-actin staining was performed to assess actin reorganization. Results: PolyIC induced the expression of RIG-I and MDA5 in dose- and time-dependent manner, accompanied with interferon-β (IFN-β) and interleukin-6 (IL-6) up-regulation and actin reorganization. Temporal knockdown of RIG-I by siRNA decreased IFN-β expression, while MDA5 siRNA inhibited IFN-β and IL-6 expression. Actin reorganization was attenuated by RIG-I and MDA5 knockdown. Conclusion: RIG-I and MDA5 may play a role in the antiviral responses of podocytes. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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