| Abstrakt: |
This study was carried out in the Laboratory of Plant Phytology at the Department of Plant Protection at Faculty of Agriculture, University of Karbala, in order to isolate and diagnose twelve isolates of Fusarium spp. isolated from imported banana fruits that found in local markets. The fungal isolates were identified using polymerase chain reaction (PCR) and nucleotide sequencing of DNA multiples using ITS1 and ITS4. The results of the analysis of the sequence of the nitrogen bases of the double-stranded PCR products using the Basic Local Alignment Search Tool (BLAST) showed that all the isolates of the fungus are attributed to Fusarium Spp. The Nucleotide sequence analysis of isolation isolates in this study showed that there was a genetic variation among them, as well as between newly recorded isolates and other isolates belonging to the same fungus, which were established at the National Center for Biotechnology Information, NCBI). The results showed that nine isolate isolates were not previously registered with the National Center for Biotechnology Information (NCBI), so they were registered under entry numbers (MN093942, MN121160, MN121161, MN121533, MN132885, MN121544, MN123531, MN121577 and MN121630). [ABSTRACT FROM AUTHOR] |
