| Autor: |
Washizu, Masao, Kimura, Yuji, Kobayashi, Takuya, Kurosawa, Osamu, Matsumoto, Sayoko, Mamine, Takayoshi |
| Předmět: |
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| Zdroj: |
AIP Conference Proceedings; 2004, Vol. 725 Issue 1, p67-76, 10p |
| Abstrakt: |
The high-specificity self-assembling nature of DNA makes the molecule a candidate for the template for the construction of molecular devices. In order to construct a functional device, the template must be positioned onto a predetermined site on a substrate to allow external connections, and the components must be properly aligned onto the template. A key factor is the high yield of binding, especially when the device consists of many components. Such high yield requires that the bases of the template DNA be exposed so that its counterpart can interact freely, and the template be stretched to avoid folding or coiling that hampers the interaction. We have developed electrokinetic DNA manipulation method, by which a double-stranded DNA is stretched and immobilized bridging over an electrode pair, with the molecular ends anchored while the middle part is left free to interact with foreign molecules. However, double-stranded DNA has a closed structure and the bases inside are not easily accessible. To have a template DNA with accessible bases, two methods are developed in this paper; one being the direct stretching of single-stranded DNA, and the other being the use of a recombination protein to make the bases of double-stranded DNA accessible. The former has an advantage in its simplicity, and the latter in its mechanical stability. We expect that these stretch-and-positioned DNA with accessible base-pairs will lead to the high-yield molecular construction. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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