| Autor: |
M. A., GRIGORYAN, A. A., SIMONYAN, A. E., SIMONYAN, V. P., KHACHATRYAN |
| Předmět: |
|
| Zdroj: |
New Armenian Medical Journal; Mar2020, Vol. 14 Issue 1, p94-101, 8p |
| Abstrakt: |
Tissue engineering holds a promise to transform transplantation medicine and drug testing. One of its most promising approaches is to grow new organs by seeding decellularized tissue with autologous cells. Here we aimed to compare the methods to reseed decellularized liver scaffold with isolated hepatocytes from rats of different ages. To decellularize adult rat liver it was perfused with 1% Sodium dodecyl sulfate via portal vein. After decellularization, perfusion with Phenol Red was used to confirm the integrity of hepatic vessels. Rat hepatocytes were isolated from neonatal (1-2 day old), 6-week and 10-week old rats. Three-dimensional distribution of viable cells within the recellularized scaffold was monitored using confocal microscopy by loading cells with CellTracker Red CMTPX. Metabolic activity of engrafted hepatocytes was examined using Bradford assay for albumin quantification. Finally, histological analysis of control, decellularized and recellularized samples was performed. Perfusion-based decellularization protocol successfully cleared the liver from most of the cellular content. Diminished amount of cells in 1% Sodium dodecyl sulfate perfused liver samples was confirmed by Coomassie blue staining and histology. After five days of culture, hepatocytes from neonatal rats proliferated, migrated within scaffold material. In contrast, cells from 6 and 10-week-old animals exhibited a lack of proliferation and poor adhesion. Engrafted hepatocytes from neonatal rats had significantly higher rates of albumin secretion as compared to their aged counterparts. We have implemented liver decellularization and recellularization protocols using hepatocytes from rats of different ages. The data showed significant dependence of cell attachment and proliferation rates on the age of donor animal. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
