Evaluation of a Liquid Biopsy Protocol using Ultra-Deep Massive Parallel Sequencing for Detecting and Quantifying Circulation Tumor DNA in Colorectal Cancer Patients.

Autor:	Nguyen, Huu Thinh, Tran, Duc Huy, Ngo, Quoc Dat, Pham, Hong-Anh Thi, Tran, Thanh-Truong, Tran, Vu-Uyen, Pham, Truong-Vinh Ngoc, Le, Trung Kien, Le, Ngoc-An Trinh, Nguyen, Ngoc Mai, Vo, Binh Thanh, Nguyen, Luan Thanh, Nguyen, Thien-Chi Van, Bui, Quynh Tram Nguyen, Nguyen, Huu-Nguyen, Luong, Bac An, Le, Linh Gia Hoang, Do, Duc Minh, Do, Thanh-Thuy Thi, Hoang, Anh Vu
Předmět:	TISSUE analysis ALLELES BODY fluid examination COLON tumors DNA GENETIC techniques MEDICAL protocols METASTASIS GENETIC mutation RECTUM tumors HIGH throughput screening (Drug development) DESCRIPTIVE statistics SEQUENCE analysis
Zdroj:	Cancer Investigation; Feb2020, Vol. 38 Issue 2, p85-93, 9p
Abstrakt:	The identification and quantification of actionable mutations are critical for guiding targeted therapy and monitoring drug response in colorectal cancer. Liquid biopsy (LB) based on plasma cell-free DNA analysis has emerged as a noninvasive approach with many clinical advantages over conventional tissue sampling. Here, we developed a LB protocol using ultra-deep massive parallel sequencing and validated its clinical performance for detection and quantification of actionable mutations in three major driver genes (KRAS, NRAS and BRAF). The assay showed a 92% concordance for mutation detection between plasma and paired tissues and great reliability in quantification of variant allele frequency. [ABSTRACT FROM AUTHOR]
Databáze:	Complementary Index
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