| Autor: |
Martino, N. A., Marzano, G., Mastrorocco, A., Lacalandra, G. M., Vincenti, L., Hinrichs, K., Dell'Aquila, M. E. |
| Předmět: |
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| Zdroj: |
Reproduction, Fertility & Development; 2019, Vol. 31 Issue 12, p1862-1873, 12p |
| Abstrakt: |
Time-lapse imaging was used to establish the morphokinetics of equine embryo development to the blastocyst stage after in vitro oocyte maturation (IVM), intracytoplasmic sperm injection (ICSI) and embryo culture, in oocytes held overnight at room temperature (22–27°C; standard conditions) before IVM. Embryos that developed to the blastocyst stage underwent precleavage cytoplasmic extrusion and cleavage to the 2-, 3- and 4-cell stages significantly earlier than did embryos that arrested in development. We then determined the rate of blastocyst formation after ICSI in oocytes held for 2 days at either 15°C or room temperature before IVM (15-2d and RT-2d treatment groups respectively). The blastocyst development rate was significantly higher in the 15-2d than in the RT-2d group (13% vs 0% respectively). The failure of blastocyst development in the RT-2d group precluded comparison of morphokinetics of blastocyst development between treatments. In any condition examined, development to the blastocyst stage was characterised by earlier cytoplasmic extrusion before cleavage, earlier cleavage to 2- and 4-cell stages and reduced duration at the 2-cell stage compared with non-competent embryos. In conclusion, this study presents morphokinetic parameters predictive of embryo development in vitro to the blastocyst stage after ICSI in the horse. We conclude that time-lapse imaging allows increased precision for evaluating effects of different treatments on equine embryo development. In vitro embryo production is an important equine reproductive technology. Using time-lapse monitoring, we defined the kinetics of in vitro equine blastocyst development and found that holding oocytes at 25°C for 2 days decreased blastocyst production, whereas holding them at 15°C affected embryo morphokinetics, but not the blastocyst rate. Defining morphokinetic parameters via time-lapse monitoring will allow more precise evaluation of the effects of embryo treatment. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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