Evaluation of the neuromuscular junction in a middle-aged mouse model of congenital myasthenic syndrome.

Autor: Leite Schetino, Luana Pereira, Fonseca, Matheus, Magalhães Gomes, Matheus Proença Simão, Costa Valadão, Priscila Aparecida, Camargo, Wallace Lucio, Rodrigues, Hermann Alecsandro, Andrade, Jéssica Neves, Arantes‐Costa, Fernanda Magalhães, Naves, Lígia Araujo, Prado, Carla Máximo, Prado, Vânia Ferreira, Prado, Marco Antônio Máximo, Guatimosim, Cristina, Fonseca, Matheus de Castro, de Camargo, Wallace Lucio, Arantes-Costa, Fernanda Magalhães, de Castro Fonseca, Matheus
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Zdroj: Muscle & Nerve; Dec2019, Vol. 60 Issue 6, p790-800, 11p
Abstrakt: Introduction: Reduced expression of the vesicular acetylcholine transporter (VAChT) leads to changes in the distribution and shape of synaptic vesicles (SVs) at neuromuscular junctions (NMJs), suggesting vesicular acetylcholine (ACh) as a key component of synaptic structure and function. It is poorly understood how long-term changes in cholinergic transmission contribute to age- and disease-related degeneration in the motor system.Methods: In this study we performed confocal imaging, electrophysiology, electron microscopy, and analyses of respiratory mechanics of the diaphragm NMJ components in 12-month-old wild-type (WT) and VAChTKDHOM mice.Results: Diaphragms of NMJs of the VAChTKDHOM mice were similar to those in WT mice in number, colocalization, and fragmentation of pre-/postsynaptic components. However, they had increased spontaneous SV exocytosis, miniature endplate potential frequency, and diminished MEPP amplitude. No impairment in respiratory mechanics at rest was observed, probably due to the large neurotransmission safety factor of the diaphragm.Discussion: The present findings help us to understand the consequences of reduced ACh release at the NMJs during aging. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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