| Abstrakt: |
Objective To explore the therapeutic effect of curcumin on neronal apoptosis, autophagy and neural renovation in cerebral cortex of mice after traumatic brain injury (TBI). Methods A total of 90 mice were randomly divided into 4 groups: blank control group (N=12), sham group (N=26), TBI+NaCl group (N=26, NaCl was pretreated by intraperitoneal injection 30min after TBI) and TBI+ curcumin group (N=26, curcumin was pretreated by intraperitoneal injection 30 min after TBI). Propidium Iodide (PI)-labeled cell-counting was used to study the cell protective effect of curcumin. Expression of apoptosis and autophagy related proteins B celllymphoma (Bcl-2), activated Caspase-3, P62, microtubule-associated protein 1 light chain 3 (MAP1LC3-II) was detected by Western Blotting. The lesion volume of cortex was tested by Cavalieri mothod. Results PI positive cells significantly increased 48h after TBI ( t = 26.000, P = 0.000; t = 18.520, P = 0.000), and Reduced after curcumin injection ( t =3.686, P = In cortex lesion area, expression level of apoptosis associated protein Bcl-2 decreased ( t = 5.556, P = 0.000; t = 3.254, P = 0.0088; t = 4.485, P = 0.001; t = 3.150, P = 0.009) ), expression level of activated Caspase-3 increased ( t =8.534, P =0.000; t =3.468, P =0.005; t =8.565, P =0.000; t =2.846, P =0.016), expression level of autophagy associated protein P62 decreased ( t =3.574, P =0.004; t =7.612, P =0.000; t =3.465, P = 0.005; t =8.637, P =0.000), expression level of MAP1LC3-II increased ( t =7.101, P = 0.000; t = 7.656, P = 0.000; t = 6.816, P = 0.000; t = 9.043, P = 0.000), curcumin suppressed apoptotic activity by showing higher expression level of Bcl-2 ( t = 3.290, P = 0.007), Lower expression level of activited Caspase-3 ( t =3.520, P = 0.005), lower expression level of P62 ( t =3.595, P =0.004), and higher expression level of MAP1LC3-II ( t = 4.954, P =0.000) More tissue damage was seen 28d after TBI ( t =34.813, P =0.000; t =11.172, P =0.000) but less tissue damage was seen in TBI+curcumin group ( t =4.525, P =0.003). Conclusions Curcumin could inhibit neuronal apoptosis and enhance antophagic activity., and it may induce neuroprotection and promote nerual renovaion after fraumatic brain injury. [ABSTRACT FROM AUTHOR] |
