| Autor: |
Sommer-Lassa, Morgan M, Reecy, James, Severin, Andrew, Somwarpet-Seetharam, Arun, Mayes, Mary Sue |
| Předmět: |
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| Zdroj: |
Journal of Animal Science; 2019 Supplement, Vol. 97, p40-41, 2p |
| Abstrakt: |
Antimicrobial resistance (AMR) is a growing worldwide health threat. Increases in AMR are preventing the success of therapeutic treatment(s), which leads to diminished animal well-being. Innovative research is needed to explore alternatives (i.e. feed supplementation) to reduce the dependencies of antibiotics in livestock production. Diet composition has previously been shown to play a large role in altering microbial communities. The objective of this project was to evaluate the effect a novel Saccharomyces cerevisiae feed additive on the abundance and prevalence of AMR genes present in beef cattle microbiomes (nasal, deep nasopharyngeal cavities, and fecal matter). Samples were collected from weaned steers (n = 32). Steers were fed four different dietary treatments (Control, 12 g/hd/day, 18 g/hd/day, post-transportation 18/g/hd/day). Analyses performed evaluated if AMR gene abundance or prevalence was reduced due to treatment. DNA samples were extracted and whole genome shotgun sequencing was conducted by Illumina Technologies. Sequences were mapped to the UMD 3.1 Bovine reference genome and the National Database of Antibiotic Resistant Organisms (NDARO) (n2= 4,187) to annotate and quantify microbial AMR genes. Hundreds of AMR genes were detected across fecal (371), nasal (363), and deep nasopharyngeal (121) samples. Nasal and deep nasopharyngeal samples primarily aligned to the bovine genome and were not analyzed further. DNA from fecal samples aligned predominantly with microbial genes and were the primary focus of the research. Linear models and treatment contrast analyses were implemented to test for nominally significant treatment differences (P < 0.05). Dietary treatment significantly impacted AMR genes, predominantly Mef(EN2) and Lnu(AN2). On average, Lnu(AN2) and Mef(EN2) AMR gene read abundances were reduced due to dietary treatments by 57% and 55%. In conclusion, feeding this novel Saccharomyces cerevisiae feed additive did significantly reduce AMR gene read abundances. However, further research will increase our understanding of dietary treatment impacts within the fecal microbiome. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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