Ion Homeostasis during the Growth of Human Mesenchymal Stem Culture. I. Density-Dependent Changes of Intracellular K+ and Na+ Content and K+ Influxes.

Autor: Shatrova, A. N., Vinogradova, T. A., Domnina, A. P., Zemelko, V. I., Pugovkina, N. A., Nikolsky, N. N., Marakhova, I. I.
Zdroj: Cell & Tissue Biology; Mar2019, Vol. 13 Issue 2, p93-99, 7p
Abstrakt: In this study, we reported proliferation-related changes in cell K+ and Na+ content and K+ influxes in cultivated human mesenchymal stem cells (MSCs). The intracellular K+ content calculated per cell protein amount was found to decrease in the growing culture, whereas intracellular Na+ content was not significantly changed. It was also revealed that, at higher densities of an hMSC monolayer, the ouabain-sensitive K+ influx was decreased thus indicating a decline in Na+, K+ pump-mediated transport. We analyzed the cell cycle profiles of hMSC cultures and found that, under optimal culture conditions in high-density cultures, the decline in K+ content per cell protein was correlated with accumulation of G1 cells and slowing down cell proliferation in the population. It is concluded that cell K+ content per cell protein is an informative test for assessing the functional status of stem cells in vitro. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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