| Autor: |
Kazemi, Vida, Mojtahedzadeh, Mojtaba, Siadat, Seyed Davar, Hadjiakhondi, Abbas, Ameri, Abdulghani, Badi, Sara Ahmadi, Manayi, Azadeh, Bagheri, Mahdi |
| Předmět: |
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| Zdroj: |
Journal of Contemporary Medical Sciences; Jan/Feb2019, Vol. 5 Issue 1, p20-23, 4p |
| Abstrakt: |
Objective In this study the effect of Royal jelly on the growth of two important members of Bacteroides spp.; Bacteroides fragilis and Bacteroides thetaiotaomicron, was evaluated. Also the physicochemical properties and cytotoxicity effects of Royal jelly on Caco-2 cell line as gastrointestinal epithelial cell model, was assessed. Methods Bacteria, B. fragilis and B. thetaiotaomicron were grown on brain heart infusion (BHI) broth medium supplemented with Royal jelly in three different concentrations (2.5%, 5% and 10% v/v), both of the bacteria (1.5 × 108 cfu/mL) were inoculated to BHI broth contained Royal jelly in anaerobic condition. To calculate the bacterial optical density (OD), the absorbance was measured at 600 nm after an overnight. Also Caco-2 cells, was used to study the effects of Royal jelly on epithelial cell viability, and the physicochemical properties consist of total proteins, polysaccharides, phenolic compounds, total lipids, ash and moisture by UV-Vis spectrophotometric and gravimetric methods were evaluated. Results The growth of B. fragillis and B. thetaiotaomicron were increased by Royal jelly (2.5%, 5% and 10% v/v concentrations) and the results indicated that Royal jelly increased the growth of bacteria in a dose dependent manner (p < 0.001). In addition MTT assay showed more than 95% viability of Caco-2 cells treated with Royal jelly. The Iranian Royal jelly sample contains 59.01% water, 11.57% proteins, 12% lipids, 12.63% polysaccharide, and 5% mineral. Conclusion This study showed that Royal jelly has a potential effect in the preserving gut microbiota and it is suggested that Royal jelly as a complementary and alternative medicine can be used to treatment diseases are associated with gut microbiota-host interactions and immune regulating. Although we need to expand our knowledge by designing clinical trials to confirm the therapeutic effects of Royal jelly on gut microbiota modulation as a barrier function. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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