Autor: |
Kumar, Ravi, Jakhar, M. L., Sekhawat, Komal, Kumawat, Swarnlata, Burdak, Anita |
Předmět: |
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Zdroj: |
Journal of Plant Science Research; 2018, Vol. 34 Issue 2, p311-318, 8p |
Abstrakt: |
Present investigation was carried out with the objectives to optimize the effect of antioxidants on shoot proliferation for mass multiplication in different explants like nodal segment and shoot apex pomegranate (Punica granatum L.) cv. Sindhuri. Different explants viz., nodal segment and shoot apex obtained aseptically were inoculated on MS medium supplemented with responsive level (Nodal segment, 2.0 mg/l BAP and shoot apex, 2.5 mg/l BAP) of plant growth regulators for shoot multiplication. Antioxidants viz: activated charcoal, ascorbic acid, citric acid and polyvinylpyrollidone were used to control the accumulation of phenolic compounds in the culture medium and enhance the rate of micropropagation. The cultures were incubated at 25±2°C under 14:10 hour's photoperiod with a light intensity of 3000 lux. Activated charcoal (200 mg/l) was found best antioxidant for maximum shoot bud induction in nodal segment and shoot apex explant through controlling of phenolic compounds. [ABSTRACT FROM AUTHOR] |
Databáze: |
Complementary Index |
Externí odkaz: |
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