Metabolomics evaluation of early-storage red blood cell rejuvenation at 4°C and 37°C.

Autor:	Gehrke, Sarah, Srinivasan, Amudan J., Culp‐Hill, Rachel, Reisz, Julie A., Ansari, Andrea, Gray, Alan, Landrigan, Matthew, Welsby, Ian, D'Alessandro, Angelo, Culp-Hill, Rachel, D'Alessandro, Angelo
Předmět:	REJUVENATION ERYTHROCYTES BLOOD transfusion METABOLOMICS SICKLE cell anemia ADENOSINE triphosphate metabolism BIOCHEMISTRY BLOOD collection BLOOD banks COMPARATIVE studies ENERGY metabolism GLYCOLYSIS RESEARCH methodology MEDICAL cooperation OXIDATION-reduction reaction RESEARCH TEMPERATURE EVALUATION research ACYCLIC acids
Zdroj:	Transfusion; Aug2018, Vol. 58 Issue 8, p1980-1991, 12p
Abstrakt:	Background: Refrigerated red blood cell (RBC) storage results in the progressive accumulation of biochemical and morphological alterations collectively referred to as the storage lesion. Storage-induced metabolic alterations can be in part reversed by rejuvenation practices. However, rejuvenation requires an incubation step of RBCs for 1 hour at 37°C, limiting the practicality of providing "on-demand," rejuvenated RBCs. We tested the hypothesis that the addition of rejuvenation solution early in storage as an adjunct additive solution would prevent-in a time window consistent with the average age of units transfused to sickle cell recipients at Duke (15 days)-many of the adverse biochemical changes that can be reversed via standard rejuvenation, while obviating the incubation step.Study Design and Methods: Metabolomics analyses were performed on cells and supernatants from AS-1 RBC units (n = 4), stored for 15 days. Units were split into pediatric bag aliquots and stored at 4°C. These were untreated controls, washed with or without rejuvenation, performed under either standard (37°C) or cold (4°C) conditions.Results: All three treatments removed most metabolic storage by-products from RBC supernatants. However, only standard and cold rejuvenation provided significant metabolic benefits as judged by the reactivation of glycolysis and regeneration of adenosine triphosphate and 2,3-diphosphoglycerate. Improvements in energy metabolism also translated into increased capacity to restore the total glutathione pool and regenerate oxidized vitamin C in its reduced (ascorbate) form.Conclusion: Cold and standard rejuvenation of 15-day-old RBCs primes energy and redox metabolism of stored RBCs, while providing a logistic advantage for routine blood bank processing workflows. [ABSTRACT FROM AUTHOR]
Databáze:	Complementary Index
Externí odkaz:	Zobrazit plný text záznamu Plný text