| Abstrakt: |
Alkaline lipases are one of the most important industrial enzymes that have several uses in detergents, food, pharmaceutical, cosmetics, textile, leather and biomedical industries. For screening the lipase-producing bacteria, olive agar A, B, C and Rhodamine B agar were used. The best environmental conditions and their interactions for alkaline lipase production were obtained using Taguchi statistical test. Enzyme purification was carried out by ammonium sulfate precipitation, dialysis and SDS-PAGE, respectively. The best alkaline lipase-producing strain, WS3, isolated from Persian Gulf, was named Marinobacter alkaliphilus ABN-IAUF-1 (GenBank accession number: KP403723). The maximum enzyme activity of 37.00 (μ/ml) was measured in the presence of coconut oil as the carbon source in oil broth medium after 48-h incubation at the pH 8.00 and 30 °C. The most effective factors in enzyme production were nitrogen and carbon sources with 32 and 25% of influence, respectively. Precipitation and dialysis increased the enzyme purity 1.90-2.33 times. The total enzyme activity of 925.00 ± 20.00 U in the oil broth, 96.00 ± 7.00 U after precipitation with ammonium sulfate and 60.00 ± 5.00 U after dialysis was measured. The molecular weight of alkaline lipase was measured as 55 kDa. In conclusion, this is the first report of production of alkaline lipase by a halo-alkalophilic bacterium, Marinobacter alkaliphilus ABN-IAUF-1, that was obtained from Persian Gulf, Iran. The application of enzymes produced by extremophiles such as Marinobacter alkaliphilus could be an asset in the modern environmental microbiology as well as food and industrial biotechnology. [ABSTRACT FROM AUTHOR] |
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