| Autor: |
van Belzen, Nico, Diesveld, Michaël P., Angelique C. J. van der Made, Nozawa, Yoshihiro, Dinjens, Winand N. M., Vlietstra, Remko, Trapman, Jan, Bosman, Fred T. |
| Předmět: |
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| Zdroj: |
European Journal of Biochemistry; 12/15/95, Vol. 234 Issue 3, p843-848, 6p |
| Abstrakt: |
To investigate the working hypotheses that stem cells or their early descendants are prime targets for neoplastic transformation, and that the degree to which a neoplasm retains the immature phenotype is an important determinant of tumor aggressiveness, we have identified several mRNAs that are downregulated during the in vitro differentiation of HT29D-D4 colon carcinoma cells. These genes include heat-shock cognate protein Hsc70, adenylosuccinate lyase, B23/nucleophosmin, α-tubulin, and a novel gene designated DS-1. The DS-1 mRNA has a length of approximately 0.9kb and is downregulated 4.7-fold upon differentiation. From DS-1 cDNA, a protein of 206 amino acids with a molecular mass of 24kDa and an isoelectric point of 10.9 can be deduced. An antiserum directed against a synthetic peptide detected a minor band of the expected size in Western blots, as well as major band of lower size that may represent a processed form of the protein. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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