| Autor: |
Liming YUAN, Nan MA, Jiaohuan CAO, Yi WEN, Xiangguang LIU, Xianxian ZHOU, Shuwen KUANG, Mengjie YANG, Wanxin OUYANG, Shijie JIA, Haibin WANG, Xiaojun TAO, Zhaojun ZENG |
| Předmět: |
|
| Zdroj: |
Medicinal Plant; Aug2017, Vol. 8 Issue 4, p52-61, 4p |
| Abstrakt: |
[Objectives] To investigate the mechanism of DNA damage of cisplatin (DDP), a broad spectrum anticancer drug on breast cancer MCF-7 cells, and to study the mechanism of apoptosis induced by DDP. [Methods] MCF-7 cells were treated by DDP (0 mg/L, 2 mg/L, 4 mg/L, 6 mg/L, 6 mg/L, and 10 mg/L) for 48 hours. MTT assay was used to detect the inhibitory effect of DDP on MCF-7 cells and IC50 value was calculated. Western blot was adopted to detect the expression of γH2AX, which was the marker of DNA double stranded breaks (DSBs) and ATM (sensory molecules of DSBs), the apoptotic signal transduction molecule cleaved easpase-3, and the proteins associated with apoptosis calpain. [Results] DDP inhibited MCF-7 cell activity in a concentration-dependent manner and IC50 was 7. 57 mg/L. In contrast to the control group (without DDP treatment), MCF-7 cells with DDP treatment expressed more γH2AX, ATM, cleaved easpase-3 and calpain. [Conclusions] DDP could inhibit the activity of breast cancer MCF-7 cells. Its mechanisms may be associated with inhibition of MCF-7 cell apoptosis, induction of DNA double strand breaking and the expression of pro-apoptotic protein up-regulation. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
