| Abstrakt: |
Separation of echioidinin-5-O-β-D-glucopyranoside (ECG) from Andrographis species of plants by column chromatographic techniques had various shortcomings. Therefore, we report a rapid and efficient method to isolate ECG from ethyl acetate fraction of petroleum ether defatted methanol extract of Andrographis echioides (AE) aerial parts. The isolated and purified ECG was identified by melting point, UV, FTIR, ¹H- and 13C-NMR and mass spectroscopy studies and used to develop a simple and reproducible HPTLC method for its determination in AE. The method was validated for linearity, precision, robustness, specificity, accuracy, and limits of detection and quantification. The method employed TLC aluminum plates precoated with silica gel 60 F254 as the stationary phase and chloroform-methanol (77.5:22.5, v/v) as a mobile phase. A CAMAG TLC Scanner 3 was used for spectrodensitometric scanning and analysis at 254 nm. The linear regression analysis data for the calibration plot shows good linearity relationship with r² = 0.9940, in the concentration range of 200-1400 ng per spot. The average percentage recovery of ECG from methanol extract was 96.69 ± 0.645 and its content in the methanol extract of aerial parts of AE was 0.925% (dry weight). The limits of detection and quantification were 54.372 and 68.466 ng, respectively. This method can be used for routine quality control analysis of the various Andrographis species containing ECG. [ABSTRACT FROM AUTHOR] |
Full text from SpringerLink