| Autor: |
Motheo, TF, Arnold, DR, Padilha‐Nakaghi, LC, Pires‐Buttler, EA, Alves, AE, Apparicio, M, Vicente, WRR, Lopes, FL |
| Předmět: |
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| Zdroj: |
Reproduction in Domestic Animals; Apr2017 Supplement, Vol. 52, p103-107, 5p |
| Abstrakt: |
Contents Post-translational modifications of histones, such as acetylation, are involved in regulating chromatin remodelling and gene expression. Proper in vitro maturation ( IVM) of canine oocytes, for many reasons, is up to now inefficient. This study aimed to evaluate the post-translational histone H4 acetylation at lysine 5 (H4K5) in immature and post- IVM canine oocytes. Oocyte nuclear stage was assessed using Hoechst 33342 staining. Acetylation patterns were determined by indirect immunofluorescence staining of immature and post- IVM oocytes, using an antibody against the acetylated lysine 5 residue on histone 4 (H4K5ac). The experiment was repeated four times, with a total of 7-17 oocytes evaluated per stage. Immunofluorescence signal was quantified using the NIH imagej software. Data were expressed as a percentage of the average fluorescence intensity of the specific antibody over the intensity of DNA, as determined by Hoescht staining. H4K5ac displayed a significantly higher acetylated pattern in immature oocytes (0.97 ± 0.08) when compared to post- IVM oocytes at different nuclear stages. There was a decrease in the fluorescence level of the matured oocytes with the progression of meiosis ( GVBD: 0.47 ± 0.06 and MI/ MII: 0.35 ± 0.04). Similarly to other domestic species, we hypothesized that post-translational modification of histone acetylation takes place during meiosis of in vitro matured canine oocytes. However, it remains to be investigated whether these changes occur during in vivo maturation. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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